WebFeb 26, 2024 · This work identified and characterized proteins interacting with partitioning protein ParB and mapped the domain of interactions with its cognate ParA partner and showed that ParB–ParA interactions are crucial for the chromosome segregation and for proper SMC action on DNA. View 1 excerpt, cites background WebMay 1, 2007 · Gene expression in areas near parS sites was similar in wild type and a spo0J null mutant, indicating that binding and spreading of Spo0J on DNA does not normally silence transcription of nearby genes. We investigated the genome‐wide DNA binding of the chromosome partitioning and sporulation protein and ParB family member Spo0J in …
Crosstalk Regulation Between Bacterial Chromosome Replication …
WebHow sister chromosomes partition to opposite ends of the predivisional cell is a central and unresolved aspect of the bacterial cell cycle (reviewed in Gordon and Wright, 2000; Draper and Gober, 2002). For many years chromosome partitioning was thought to be a passive process relying on zonal membrane growth between two chromosome … WebNov 2, 2024 · A necessary f eature for correct chromosome partitioning comes from a particular ability of ParB . proteins to clamp the parS-DNA as a dimer (4, 5) and to subsequently slide along the DNA by ... how is ultraviolet light helpful
Characterization of a prokaryotic SMC protein involved in …
WebDec 31, 2024 · Consistency of the chromosome partitioning. In order to check the robustness of our chromosome partitioning method, the same analysis was performed on two biological replicates of the Hi-C data set , and the consistency between partitioning schemes across data sets was tested using mutual information. Briefly, to be consistent, … The parABS system is a broadly conserved molecular mechanism for plasmid partitioning and chromosome segregation in bacteria. Originally identified as a genetic element required for faithful partitioning of low-copy-number plasmids, it consists of three components: the ParA ATPase, the ParB DNA-binding protein, and the cis-acting parS sequence. The parA and parB genes are typically found in the same operon, with parS elements located within or adjacent to this operon… WebJun 16, 2024 · Schematics of chromosome replication and segregation in bacteria. (A) A mother chromosome (black) with an origin (ori) and terminus (ter) of replication site resides in the cell. Inset shows the … how is uluru being protected