Gfp transgenic n. benthamiana 16c
WebOct 2, 2006 · To create a similar model for studies of glial cells in zebrafish, we have generated transgenic lines of zebrafish, Tg ( gfap: GFP), that express GFP driven by … WebJan 4, 2024 · GFP expression was equivalent to 16C in a two-copy event. Local GFP silencing was observed in all transgenic and 16C hemizygous lines after topical …
Gfp transgenic n. benthamiana 16c
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WebJan 14, 2024 · When we inoculated the green fluorescent protein (GFP)-expressing transgenic Nicotiana benthamiana (16c) with the CMV-A1 constructs containing various sizes of the 35S promoter sequences, we found that the GFP expression levels were downregulated due to RdDM and that the sizes of the virus-integrated sequences were … WebDec 15, 2024 · The 16c line transgenic N. benthamiana plants carrying a GFP transgene have been used extensively to study RNA silencing and VSR [ 23 ]. The plants of this transgenic line accumulate a high amount of GFP mRNA and emit green fluorescence under UV lamp.
WebMar 15, 2024 · The GFP16C-like systemic silencing phenotype was only observed in the two-copy line. The partial transposase had no impact on transgene expression level, … WebIn this study, we identified a new citrus vein enation virus (CVEV) isolate (named CVEV-DT1) through sRNA high-throughput sequencing and traditional sequencing. Phylogenetic analysis based on whole genome sequences of all known CVEV isolates revealed that CVEV-DT1 was in an evolutionary branch with other isolates from China. Molecular …
WebApr 28, 2016 · Transgenic N. benthamiana plants expressing green fluorescent protein (GFP, line 16c) were infiltrated with a mixture of Agrobacterium harboring 35S-GFP and either a test or a control construct. The construct 35S-P19 or empty vector (pCHF3) containing no insert were used as positive and negative controls, respectively. WebdsRNAs targeting GFP leads to a systemic silencing response in N. benthamiana. N. benthamiana is model dicot that has been widely adopted by public and private sector …
WebAug 11, 2024 · Both GFP-transgenic (line 16c) and wild-type N. benthamiana plants were grown in a growth chamber at 25/22 °C day/night temperature and 16/8 h light/dark regime for 4–5 weeks. The expanded leaves of the N. benthamiana 16c plants were used for silencing suppression studies following the agro-infiltration protocol described in Patil & …
WebMar 28, 2024 · Furthermore, agroinfiltration assays in N. benthamiana wilt type or GFP-transgenic 16c indicated that ToCV CPm protein effectively suppressed local RNA silencing induced by single-stranded but not double-stranded RNA, which probably resulted from the activity of binding double-stranded but not single-stranded RNA by ToCV CPm protein. hs bremen studiengang bwWebMar 1, 2024 · Green fluorescent protein (GFP) transgenic N. benthamiana plants (line 16c) [11] was used in the present study and grown from seeds in a growth chamber under 16 h light and 8 h dark photoperiod at 25 °C. Healthy leaves of six-week-old plants were used for agroinfiltration experiment. hs bursaryWebtransgenic line has been used more extensively than any other. This line, 16c, expresses the Aequorea victoria green fluorescent protein (GFP), highly and constitutively, and … autun pyramideWebFeb 23, 2024 · benthamiana 16c are likely to be deeper and more accurate with a greater knowledge of the GFP gene integration site. Therefore, using next generation … hs certifikatWebMar 28, 2024 · Furthermore, agroinfiltration assays in N. benthamiana wilt type or GFP-transgenic 16c indicated that ToCV CPm protein effectively suppressed local RNA silencing induced by single-stranded but not double-stranded RNA, which probably resulted from the activity of binding double-stranded but not single-stranded RNA by ToCV CPm protein. autun tympanumWebS7 Fig: Examination of viral suppressors of RNA-silencing (VSR) in RTIV.(A) Schematic representation of the Agrobacterium infiltration.(B) 16C transgenic N. benthamiana were respectively co-infiltrated with GFP and P0, CP, MP or full length of RTIV. Empty vector and VSRs were used as negative and positive control, respectively. GFP was visualized … autun moulinsWebApr 23, 2003 · A transgenic N. benthamiana line (GFP-16c) carrying a 35S-GFP transgene with a kanamycin-selectable marker at a single locus in homozygous condition (Ruiz et al., 1998) and a transgenic A. thaliana ecotype C24 line (Dalmay et al., 2000) carrying a similar transgene were used for pX7-GFPi transformation. hs butyl tape