WebThe loss of responsiveness in these mice ( Goldenticket ) was due to a destabilizing point mutation in STING. We isolated fibroblasts from wild-type and Goldenticket mouse lungs and tested their ... WebFeb 1, 2011 · Here we describe a novel mutant mouse strain, Goldenticket (Gt), that fails to produce type I IFNs upon L. monocytogenes infection. By genetic mapping and …
A Human Gain-of-Function STING Mutation Causes …
WebSep 25, 2011 · Mouse Sting and the goldenticket (I199N) mutant allele of Sting were cloned into pcDNA3 with a carboxy terminal hemagglutinin (HA) tag as described previously 11. DNA encoding the carboxy terminal domain of mouse Sting (nucleotides 414 - 1137) was cloned into pET28a for recombinant protein expression in E. coli. WebAug 19, 2024 · Extended Data Fig. 1 DCs and T cells develop normally in C9orf72−/− mice, but markers of immune activation and inflammatory cytokine production are seen from a young age. a, Percentage of DC ... effectiveness of flu vaccine 2023
STING and TRIF Contribute to Mouse Sepsis, Depending on... : …
WebApr 4, 2024 · Mice. C57BL/6 (WT) and “Goldenticket” Sting gt (Jackson stock #017537) adult mice were obtained from Jackson Laboratory. CD1 mice were from Charles River. Animal usage in this project followed guidelines put forth by the Johns Hopkins University Animal Care and Use Committee (ACUC). The procedures are part of our ACUC … WebThe N-ethyl-N-nitrosourea-induced Goldenticket mouse mutant reveals an essential function of sting in the in vivo interferon response to Listeria monocytogenes and cyclic … WebMar 1, 2024 · There were no significant differences in outcomes between in-house bred and purchased mice. Goldenticket (Gt) STING-deficient mice in the C57BL/6 J background were purchased from Jackson Laboratories and bred in-house (Sauer et al., 2011). IRF-3-/-mice in the C57BL/6 J background were bred in-house (Sato et al., 1998, Murphy et al., … effectiveness of fiber supplements