WebApr 8, 2024 · GST pull-down. E. Coli derived GST-Vector or GST-PRDX1 fusion protein was purified and prepared by Proteintech (Wuhan, China). Briefly, the total protein of 293T cells with or without Flag-tagged IRAK1 overexpression was incubated with GST-PRDX1 or GST-Vector fusion protein at 4 °C overnight. Then, cell lysates were incubated with … WebThe pFN2A and pFN2K (GST) Flexi® Vectors are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase …
Addgene: pcDNA3-C-terminal GST
WebBacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P-3. Sequence Author : GE Healthcare WebAug 4, 2015 · Two recombinant proteins fused with glutathione S-transferase at their N -terminuses, namely GST- NvKSPI-1 and GST- NvKSPI-2, were successfully detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with molecular weights of about 33 kDa and 36 kDa, respectively. 15毫升是多少盎司
Purification of proteins fused to glutathione S-transferase
WebGST Expression Vector Product Specification Sheet Code: 28-9545-49 Warning ... Expression of GST fusion proteins can be monitored using the Anti-GST Antibody (27 … WebMay 30, 2024 · Glutathione-S-transferase (GST) fusion protein expression vectors are used widely for the high-level expression and subsequent purification of proteins in Escherichia coli (1,2).Glutathione Sepharose-bound GST fusion proteins can be eluted competitively by using reduced glutathione without affecting the antigenicity and … WebExpression and purification of the HPV58 E6E7-GST fusion protein. The HPV58 E6E7 fusion fragment was amplified by polymerase chain reaction. The pET-42a(+) prokaryotic expression vector and the HPV58 E6E7 fragment were both cut with EcoR I and Xho I and then linked together to produce the recombinant plasmid pET-42a(+)-HPV58 E6E7. 15毫升有多少