Web11 nov. 2024 · To fully differentiate into hepatocyte-like cells and reach optimal expression of metabolism enzymes, HepaRG cells are conventionally cultured for 14 days in the presence of DMSO after an initial 14-day period of proliferation to achieve high-density confluence (Aninat et al. 2006; Cerec et al. 2007 ). WebWe’ve developed a cost-effective genetic toxicology screening program that uses non-GLP approaches — a computational assessment of QSAR for genotoxicity, a mini Ames test and micronucleus assay — which can be used prior to our GLP-compliant, validated genetic toxicology test battery.
Performance of comet and micronucleus assays in metabolic
Web18 aug. 2024 · Human HepaRG™ cell cultures were exposed to increasing concentrations of 12 test chemicals in parallel 96-well plates (four test chemicals per plate) for … Web3D hepatotoxicity assay using HepaRG spheroids; 3D brain microtissue combined with high-content-imaging end points; Mitochondrial Toxicity. Functional mitochondrial toxicity assay ... In vitro micronucleus test (HCS CHO-K1 cells) In vitro micronucleus test (Flow cytometry TK6 cells) pH3 and pH2AX genotoxicity assay; brakes making a screeching sound
Kelley (Owens) Hayes - Study Design Lead II - LinkedIn
Web1 dec. 2012 · Metabolically competent HepaRG cells have served to assess the genotoxic potential of indirect chemical mutagens using the comet and micronucleus assays and the results obtained with a limited set of compounds were promising (Josse et al., 2008, Le Hegarat et al., 2010). WebHepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal for toxicity testing. Web3 nov. 2011 · HepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal … haftcremes test stiftung warentest