Web29 jul. 2024 · Cell lines were preserved in complete Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, China), supplemented with 10% fetal bovine serum (FBS, Gibco, Australia), 100 U/ml penicillin, and 100 mg/ml streptomycin, maintained at 37°C in a humidified 5% carbon dioxide (CO 2) atmosphere. WebHuman HCC cell lines HepG2 was a gift from University of Science and Technology of China, and HCCLM3 were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China), and maintained in DMEM (Hyclone, USA) supplemented with 10% FBS (Hyclone, USA) and 2 nmol/L L-glutamine and penicillin–streptomycin.
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WebYunnan Key Laboratory of Stomatology, Kunming, China. Contribution: Conceptualization (supporting), Data curation (equal), Formal analysis (equal), Investigation (equal ... (Hyclone), 0.5% chicken embryonic extract, 4 mM L-glutamine and 1% penicillin/streptomycin) for 72 h. Then, myofibers were removed and muscle satellite … Web25 feb. 2024 · The H9c2 cells were cultured in Dulbecco′s Modified Eagle′s medium (DMEM) (Hyclone, China) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, USA) and 1% penicillin/streptomycin (Sigma, St. Louis, MO) at 37°C in a culture box, with an atmosphere of 95% air and 5% CO 2. WebHyClone Cell Boost 6 Supplement is designed to provide nutrients such as lipids, amino ... fed-batch cell culture strategies (Fig 1). Cell Boost 6 Supplement has been tested in a variety of cell lines including Chinese hamster ovary (CHO), hybridoma, NS0, human embryonic kidney (HEK) 293, and T-cells. Key features of Cell Boost 6 Supplement ... insurance book of business value