Web10 de mai. de 2024 · Purpose To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. Methods and results When OGCs were cultured with MGCs for 12 days, there were … Web24 de fev. de 2024 · Impaired oocyte viability with increasing age is the greatest rate-limiting factor to female fertility, raising the need for therapeutic interventions that maintain and/or rejuvenate oocyte function. Key mechanisms for oocyte failure include genome instability, elevated reactive oxygen species (ROS), disrupted mitochondrial activity, and …
Effects of N-acetylcysteine on Growth, Viability, and Ultrastructure …
WebA higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability. The type of CPAs and Ca2+ interfered differentially on oocyte pathways to functionality, and this should be considered when choosing a cryopreservation protocol. KW - Bovine. KW - Calcium. KW - Cryoprotectants. KW - Fatty acid composition Web12 de abr. de 2024 · Additionally, clones may display unusually high birthweights, developmental abnormalities, or low neonatal viability [6, 10]. Several lines of research attempted at improving cloning efficiency, such as choice of alternative donor cell types, improving oocyte quality, reducing the number of donor cell passages, and trying … irm rentals orlando florida
Bovine oocyte membrane permeability and cryosurvival: effects of ...
Web17 de nov. de 2024 · This study aimed to investigate the effects of different concentrations of N-acetylcysteine (NAC) on the growth, antrum formation, viability, and ultrastructure of … WebZurück zum Zitat Solé M, Santaló J, Boada M, Clua E, Rodríguez I, Martínez F, Coroleu B, Barri PN, Veiga A (2013) How does vitrification affect oocyte viability in oocyte donation cycles? A prospective study to compare outcomes achieved … WebCultured as gonadal fragments, the majority of analyses indicated similar results for stages I and II oocytes. In contrast, stage III oocytes seemed viable for not longer than 24 h. … irm riedisheim