Peak tailing in ion chromatography
WebAug 17, 2024 · Common causes of peak tailing and/or low resolution include (but are not limited to): Presence of Teflon (PTFE) in the sample flow path. Teflon is very hydrophobic, which causes proteins to absorb to it and can result in tailing issues. If a Teflon flow cell is used, switch to a stainless steel or titanium flow cell to correct this issue. WebNov 10, 2024 · A normal peak is almost symmetrical. Other chromatographic problems are identified in Basic Troubleshooting for GC Systems . Regular maintenance of your instrument in accordance with the recommended maintenance schedule will reduce the incidence of chromatography problems. Background Tailing can be caused by several …
Peak tailing in ion chromatography
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WebAug 8, 2014 · There are two main methods for defining peak tailing: Tailing factor (Tf) – widely used in the pharmaceutical industry. Let a and b be the peak half-widths at 5% of the peak height, a is the front half-width, b is the … WebAug 20, 2024 · Figure 1 Peak tailing is often seen for biomolecules due to slow secondary ionic interactions [2] ... particularly when working with ion exchange or size exclusion chromatography methods [2]. The aim of priming the system is to coat the surfaces with something other than your analyte of interest (i.e. a blocking agent). This works because …
WebThe determination of aromatic acids by ion exclusion chromatography is challenging due to peak tailing and the long retention time of hydrophobic solutes. This review discusses the retention mechanisms and the factors affecting retention, eluents and detection methods used in ion exclusion chromatography of aromatic acids such as mono-, di ... WebExample 1 – Peak Tailing. Figure 1: Chromatograms showing worsening peak tailing effects. This example shows two overlayed chromatograms in which the peak tailing is becoming more pronounced (blue chromatogram has peaks which tail more than the red chromatogram), however both are suffering from the problem.
Web4.4 Properly integrated skimmed peak and properly integrated tailing peak. 4.5 Properly integrated overload peak (i.e. benzoic acid on DB-5). ... A ion chromatographic trace of the signal for the peak used for ... (ie a peak). 6.2 Chromatography - A separation technique involving differential retention of components between stationary and ... WebFigure 1: Capillary GC chromatogram in which all analytes and the injection solvent peak show tailing to a greater or lesser degree. Sometimes peak tailing can be caused by …
WebAug 8, 2024 · Ion-exchange Chromatography - Fronting and tailing peaks. Mobile phase: 0.005M Nitric acid (HPLC grade) diluted with de-ionized water (conductivity less than 0.1 …
WebConsider using a weak exchanger if the selectivity of the strong ion exchanger is unsatisfactory, but remember that the ion exchange capacity of a weak ion exchanger varies with pH. As a result: sample loading (binding) capacity can vary with increasing pH due to loss of charge from the exchanger. goffman social action theoryWebMar 14, 2024 · In some cases, tailing can also be caused by improper sample injection techniques or sample overload. To overcome this issue, it is important to optimise the mobile phase conditions and the sample preparation techniques. A third common peak problem in HPLC is peak fronting, where the peak is asymmetric and prolonged on the … goffman stigmaWebMar 4, 2010 · In the aqueous mobile phases, the charges interactions are usually stronger and slower than the hydrophobic interactions, which produces peak tailing and long retention times. ILs can be used as mobile phase additives in reversed-phase chromatography when mixed with other solvents such as methanol and acetonitrile. goffman stigma chapter 1WebFeb 21, 2024 · The tailing is caused by the analyte molecules having different elution start times due to the injection solvent taking time to load onto the column. This phenmomena is more noticeable under isocratic conditions, where there are no focussing effects, but can to a certain extent be disguised when using gradient elution. Figure 2. goffman stigma with disabilitiesWeb14 hours ago · The analysis of complex samples is a big analytical challenge due to the vast number of compounds present in these samples as well as the influence matrix … goffman tactiful inattentionWebOne reason for this effect is that at low pH values the interactions of residual silanols with amino containing solutes are suppressed, and thus peak tailing is no longer observable. However, such low pH values may not be acceptable for the stationary phase stability. goffman stigma theory explainedWebPeak Tailing What is Happening? Compounds have not traveled through the system following a Gaussian distribution, with a bias towards extra retention/ dwell time. These … goffman study