Phospho erk antibody cst
WebAn immobilized capture antibody specific for ERK1 / ERK2 binds both phosphorylated and unphosphorylated ERK1 / ERK2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research Human/Mouse/Rat Phospho-ERK1 (T202/Y204) DuoSet … WebApr 5, 2024 · Cell lysates were then obtained and submitted to western blot analysis using anti-phospho-ERK (p-ERK) or anti-SMN antibodies. Membranes were stripped and reprobed with anti-ERK (pan-ERK) antibody ...
Phospho erk antibody cst
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Web(A) Western blot analysis of Akt (phsopho-S473 antibody) and Erk1/2 (phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) phosphorylation levels in retinas and (B) in the dorsolateral geniculate nucleus (dLGN) of the brain tissues lysates (8 weeks of chronic elevated IOP) and their densitometric quantitative analysis (* P < 0.05, ** P < 0.01, *** P < … WebApr 12, 2024 · CDK12 primary antibody (CST #11973s) was diluted in reagent dilution 1:1000, and 100 µL of the antibody dilution was added to each well in the microplate. ... Cell lysates were immunoblotted with RNA Pol II antibodies and Ser2 or-Ser5 phospho-specific RNA Pol II antibodies to determine the effective concentration of AU-16770 required to ...
WebThe Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit Anti-EGF Receptor were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System. 5 Phosphatase treatment WebPhospho-ERK Assays Extracellular signal-related kinase (ERK1/2 or p42/44) is a kinase in the mitogen-activated protein kinase (MAPK) family and phosphorylation of ERK (p-ERK) …
WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 1049 publications. Validated for WB, WB, IF, F. Available in 2 sizes. … WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody …
WebWestern blot band for Erk and phopho (p)-Erk I want to know Erk activity in my animal model - sepsis. I isolated it from liver, homogenized it, separated it by SDS-PAGE, and detected it, but...
WebThis kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels, THUNDER Format: Half-area 96-well or low-volume 384-well plate Formulation: Eu-phospho-ERK (20 uL) Acc-phospho-ERK (80 uL) Eu-Total ERK (5 uL) Acc-Total ERK (20 uL) ERK control lysate (500 uL) 5X Lysis Buffer #1 (5 mL) 10X TR-FRET Detection buffer (250 uL) Gene ... porky penguin ltdWebMay 31, 2024 · Background We recently reported that STAT1 plays a tumor suppressor role, and ERK was inversely correlation with STAT1 expression in esophageal squamous cell carcinoma (ESCC). Here, we investigated the mechanism(s) that are responsible for the ERK regulates STAT1 in ESCC. Methods We performed the immunoprecipitation (IP) to detect … sharp microwave oven yc-ms01WebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 ® Rabbit Anti-Mouse IgG Secondary Antibody at a dilution of 1/400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. sharp microwave ovens how long do they lastWebPhospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P) Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the … sharp microwave oven r395estWebUpon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. Flow Cytometry Panel Builder porky penguin cardsWebThe store will not work correctly in the case when cookies are disabled. porky on jeff\u0027s collieWebProduct overview. Abcam’s ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA kit is designed for the semi-quantitative measurement of ERK1/2 (pT202/Y204) and Total ERK1/2 protein in Human and mouse cells. The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody ... porky penguin companies house