Phospho-erk5
WebDec 1, 2010 · ERK5 is a member of the mitogen-activated protein kinase (MAPK) family that, after stimulation, is activated selectively by dual phosphorylation in the TEY motif by … WebJan 24, 2024 · Using phospho-ERK5 (T218/Y220) levels as a readout for the activation of the pathway, we exposed DU145 expressing either MEK5 or control Luciferase siRNA to different doses of IR and lysed the ...
Phospho-erk5
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WebWestern blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686) Peptide Competition: Extracts prepared from HEK293 cells transiently transfected with plasmids expressing … WebERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. For Research Use Only. Not for use …
WebMSD phosphoprotein assays provide a rapid and convenient methodfor measuring the levels of protein targets within a single, small-volume sample. The assays are available in both singleplex and multi plex formats. In a singleplex assay, an antibody for a specific protein target is coated on one electrode (or “spot”) per well. WebNational Center for Biotechnology Information
WebPhospho-ERK5 (Thr218/Tyr220) Assay. Increased signal is observed with the titration of ERK5 positive cell lysate. Signal for negative lysate remains low throughout the titration. The p Phospho-ERK5 (Thr218/Tyr220) Assay provides a quantitative measure of the data obtained with the traditional Western blot. WebApr 8, 2024 · For the Human Phospho‐Kinase Array (ref. ARY003B, R&D Systems), cell lysates of the ovarian cancer cell lines were hybridised to the array following provider's instructions for simultaneously detecting the relative levels of kinase phosphorylation sites. ... ERK5 autophosphorylation causes the appearance of a hyperphosphorylated form, …
WebSep 1, 2009 · Representative western blots to show changes in phospho-ERK5 and total ERK5 are shown in Fig. 2A (upper panels) and quantification of these is shown in Fig. 2B. Total ERK5 protein levels doubled 24 hours after the initiation of differentiation (P<0.01) and then gradually decreased during the remainder of the period investigated. By contrast ...
WebMar 10, 2024 · 研究证实,ox-ldl 与cd36 结合后可激活血小板内src、syk、plcγ2、pkc蛋白,使其发生磷酸化,进一步使nox2 激活。nox2 是血小板内介导ros 生成的关键蛋白,其活化可促进胞内产生大量的ros,进而使下游的erk5 发生磷酸化,促使血小板发生凋亡〔5,21〕 … simple country kitchenWebAnti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody 07-507 Sigma-Aldrich Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody Download Zoom This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220). rawdon creek nature reserveWebAnti-phospho-ERK5 (pThr218/pTyr220) antibody can be used in immunostaining. Rabbit anti- phospho-ERK5 (pThr218/pTyr220) antibody reacts specifically with ERK5 (88 kD) of … rawdon chutesWebAnti-phospho-ERK5 (pThr218/pTyr220) antibody produced in rabbit (Anti-BMK1); affinity isolated antibody, buffered aqueous glycerol solution; Each vial contains enough material for 10 blots; Extracellular Signal-Regulated Kinases … rawdon churchWebERK5 is a member of the mitogen-activated protein kinase (MAPK) family that, after stimulation, is activated selectively by dual phosphorylation in the TEY motif by MAPK … simple country kitchen designs layoutsWebDec 17, 2013 · ERK5 phosphorylation was assessed in the immunoprecipitate using a phosphospecific antibody. Data (mean +/- SEM of 3 independent experiments) were normalised using HA-ERK5 as loading control and expressed as fold-induction over basal conditions (*p<0.05, two-tailed T-test). simple country nailsWebERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. References (0) rawdon cc twitter