Shape assay
WebbMaryland 161 views, 9 likes, 2 loves, 29 comments, 1 shares, Facebook Watch Videos from American Academy for Oral & Systemic Health: Join us on... Webb23 sep. 2024 · Smart fluid manipulation with automatically controlled paper valves will enable automated and multi-step immunoassays on paper-based microfluidic devices. In this work, we present an integrated paper-based microfluidic platform with shape-memory polymer (SMP)-actuated fluid valves capable of automated colorimetric enzyme-linked …
Shape assay
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WebbThe SHAPE reactions were incubated at 37 °C for 22 min. The RNAs were recovered by phenol–chloroform extraction and ethanol precipitation. For XL experiments, each of the binding reactions (9 µL) was exposed to UV light (254 nm, total energy of 200 mJ/cm 2) on ice in a Stratalinker 2400 UV cross-linker (Stratagene, San Diego, CA, USA). Webb12 nov. 2024 · The first method is based on the tailing factor ( T f ). Here, you measure the peak width at 5% of the peak height above the baseline. This approach is often used in the pharmaceutical world. The other method relies on the asymmetry factor ( A s ). Here you measure at 10% of peak height above the baseline. A peak with:
WebbV_ {max} V max is the Y-value (initial rate of reaction value) at which the graph above plateaus. The substrate concentration that gives you a rate that is halfway to V_ {max} V … Webb28 aug. 2015 · For the concentration response studies and phenotypic assay, cells were plated into 96-well or 384-well, ultralow-attachment, Corning U-shaped, black clear-bottom plates (Corning 4520 and 3830, respectively) at densities of 1,500 cells/well in the appropriate media at 37°C and 5% CO 2. Media were changed every 3 days.
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WebbSHAPE-MaP sequences secondary RNA structures at various levels on a massively parallel scale. The method can be customized to interrogate small RNAs, amplicons, or rare RNA …
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